BBS Seminar Series - Eric Brown - Gut Microbiome ADP-ribosyltransferases are phage-encoded fitness factors

Schedule

Start 12 October, 2021, 11 30 AM

End 12 30 PM

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Location

Online

Via Zoom

No Province / Territory specified

Registration Required

Contact Info

Tracy Stojanovic

E-Mail: stojan@mcmaster.ca

No website provided


Bacterial ADP-ribosyltransferases (ADPRTs) have been described as toxins involved in pathogenesis through the modification of host proteins. However in our studies, we report that ADPRTs are not pathogen restricted but widely prevalent in the human gut microbiome and often associated with phage elements. To study them in-depth, we validated their biochemical activity in a large clinical isolate collection and further examined Bxa, a highly abundant ADPRT in Bacteroides. Bxa is expressed, secreted, and enzymatically active in Bacteroides and can ADP-ribosylate non-muscle myosin II proteins. Addition of Bxa to epithelial cells remodeled the actin cytoskeleton and induced secretion of inosine. Bxa-encoding B. stercoris can use inosine as a carbon source and colonizes the gut to significantly greater numbers than a bxa-deleted strain in germ-free and altered Schaedler flora (ASF) mice. Colonization correlated with increased inosine concentrations in the feces and tissues. Altogether, our results show that ADPRTs are abundant in the microbiome and act as bacterial fitness factors.

Registration Required: https://us06web.zoom.us/webinar/register/WN_3ZEXJa93Rx2HsOt1D13naA

TRAINEES: Join us! We will be hosting a virtual “Lunch and Learn” with the speaker from 12:30 – 1:00 pm.

If you are interested, please feel free to join the following link to participate:    https://us06web.zoom.us/j/89186743705?pwd=bUZ2V1dTVHl0YU5ackVQUlliRTBGUT09  


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