Demystifying Medicine Video



This video describes the novel gene-editing process known as prime editing. Prime editing is based on the CRISPR/Cas9 mechanism of gene editing. Prime editing is aimed to be a more precise version of gene editing through the use of reverse transcriptase and prime editors, thereby reducing off-target effects that can cause mutations. The video looks at the public's perspective on gene editing as well as suggested ethical solutions from recent literature.

For more information on CRISPR/Cas9 and how it works, please view this video on the McMaster Demystifying Medicine channel: What is CRISPR? And how does it work?

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References
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  • Gallagher, J. (2019, June 3). He Jiankui: Baby gene experiment was ‘foolish and dangerous’. BBC News.
  • Milova, E. (2018, November 29). Would I Want a Designer Baby? CRISPR, Gene-Editing, and You. Life Extension Advocacy Foundation.
  • Doetschman, T., & Georgieva, T. (2017). Gene Editing With CRISPR/Cas9 RNA-Directed Nuclease. Circulation Research, 120(5), 876–894. DOI: 10.1161/circresaha.116.309727
  • Cohen, J. (2019, October 25). Prime editing promises to be a cut above CRISPR. Science, 366(6464), 406. DOI:10.1126/science.366.6464.406.
  • Yang, L., Yang, B., & Chen, J. (2019, December 12). One Prime for All Editing. Cell, 179, 1448-1450. DOI:10.1016/j.cell.2019.11.030.
  • Flotte, T. R., & Gao, G. (2019). Prime Editing: A Novel Cas9-Reverse Transcriptase Fusion May Revolutionize Genome Editing. Human Gene Therapy, 30(12), 1445-1446. DOI:10.1089/hum.2019.29098.trf.
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  • Lander, E. S., Baylis, F., Zhang, F., Charpentier, E., Berg, P., Bourgain, C., ... Winnacker, E. (2019, March). Adopt a moratorium on heritable genome editing. Nature, 567, 165-168. DOI:10.1038/d41586-019-00726-5.